Aliza Amiel, PhD, Orit Reish, MD, Elena Gaber, PhD, Ronit Masterman, MD, Tally Tohami, MSc and Moshe D. Fejgin, MD
Background: While most allelic pairs of DNA replicate synchronously during the S phase of the cell cycle, some genes normally replicate asynchronously, i.e., genes on the X chromosome and imprinted genes. The replication control mechanism is unknown but was shown to be impaired in malignancies and chromosomal trisomies where replication pattern becomes asynchronous.
Objectives: To determine the level of asynchronization in replication timing of cells from patients with microdeleted genomes.
Methods: We applied monocolor fluorescent in situ hybridization with different probes on leukocytes from microdeleted genomes.
Results: All samples derived from the microdeleted genomes showed significantly higher levels of an asynchronized pattern compared to normal individuals.
Conclusions: Even a “small” genetic imbalance (microdeletion) can interfere with gene replication and cell cycle progression, as previously shown in full trisomies.
Pierre Singer, MD
Pressure sores are a well-recognized problem, with an etiology that is multifactorial and not solely a consequence of pressure itself. Malnutrition is one of the factors involved, namely low calorie and protein intake. Mainly elderly patients, patients after hip fracture, but also patients after trauma, burns or extended surgery require additional nutritional support to reduce the possibility of pressure ulcers developing. Evidence has shown the efficacy of percutaneous endoscopic gastrostomy in elderly patients with malnutrition and dementia. Nutritional support should include sufficient calories, protein, fat, carbohydrates, vitamins and minerals. Arginine is the main amino acid required and is essential for collagen deposition and wound healing. Vitamin A and zinc should be added to nutritional support.
Zvi Fireman, MD, Arkady Glukhovsky, PhD, Harold Jacob, MD, FACG, Alexandra Lavy, MD, Shlomo Lewkowicz, DSc and Eitan Scapa, MD
Mogher Khamaisi, PhD and Itamar Raz, MD
Gisele Zandman-Goddard, MD and Miri Blank, PhD
Simon Bar-Meir, MD and Eytan Bardan, MD
Imad R. Makhoul, MD, DSc, Monica Epelman, MD, Imad Kassis, MD, Marcelo Daitzchman, MD and Polo Sujov, MD
Yoram Moses, MD and Boris Yoffe, MD, FACS
Alla Shnaider, MD, Anna Basok, MD, Boris Rogachev, MD and Marcus Mostoslavsky, MD
Matitiahu Lifshitz, MD and Vladimir Gavrilov, MD
Dan Miron, MD, Yoseph Merzel, MD, Amiram Lev, MD, Jean-Jack Meir, MD and Yoseph Horowitz, MD
Ido Solt, MD, Ron Beloosesky, MD and Michael Deutsch, MD
Arie Roth, MD and Michal Golovner, MD
Carol P. Herbert, MD CCFP, FCFP