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עמוד בית
Tue, 28.05.24

Original Articles

IMAJ | volume 16

Journal 12, December 2014
pages: 774-782

Enriching Hematopoietic, Endothelial and Mesenchymal Functional Progenitors by Short-Term Culture of Steady-State Peripheral Blood Mononuclear Cells Obtained from Healthy Donors and Ischemic Patients

    Summary

    Background:

    Non-mobilized peripheral blood contains mostly committed cells with limited numbers of early progenitors.

    Objectives:

    To enrich functional progenitor cells from healthy donors and ischemic heart disease patients by short-term culture of mononuclear cells with defined culture conditions.

    Methods:

    Mononuclear cells obtained from healthy donors and ischemic heart disease patients were cultured for 7 days in a cytokine cocktail. We tested the multilineage differentiation capacities and phenotype of cultured cells.

    Results:

    The short-term culture (7 days) of all study groups with a defined cytokine cocktail resulted in two distinct cell populations (adherent and non-adherent) that differed in their differentiation capacities as well as their cell surface markers. Cultured adherent cells showed higher differentiation potential and expressed endothelial and mesenchymal fibroblast-like surface markers as compared to fresh non-cultured mononuclear cells. The non-adherent cell fraction demonstrated high numbers of colony-forming units, indicating a higher differentiation potential of hematopoietic lineage.

    Conclusions:

    This study proved the feasibility of increasing limited numbers of multipotent progenitor cells obtained from the non-mobilized peripheral blood of healthy donors and ischemic patients. Moreover, we found that each of the two enriched subpopulations (adherent and non-adherent) has a different differentiation potential (mesenchymal, endothelial and hematopoietic).

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